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Annals of Tropical Medicine & Parasitology Volume 81, 1987 - Issue 3
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Original Articles

In vitro cultivation of rickettsia-like-organisms from Glossina spp.

Susan C. WelburnTsetse Research Laboratory, University of Bristol, Langford, BristolBS18 7DU, U.K.
,
I. MaudlinTsetse Research Laboratory, University of Bristol, Langford, BristolBS18 7DU, U.K.
&
D. S. EllisLondon School of Hygiene and Tropical Medicine, Keppel Street, LondonWC1E 7HT, U.K.
Pages 331-335 | Received 18 Jun 1986, Published online: 15 Nov 2016
 
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Abstract

A method is described for the in vitro cultivation of the rickettsia-like-organisms (RLO) from Glossina spp. which are believed to be associated with susceptibility to trypanosome infection. Cultures of RLO were established by infecting a mosquito cellline (Aedes albopictus) with haemolymph taken from teneral flies. RLO from nine species of Glossina have been isolated and maintained in continuous culture using this technique.

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