A rapid staining technique for Leishmania parasites in splenic aspirate smears

C. N. ChungeDepartment of Medical Microbiology, College of Health Sciences, P. O. Box 19676, Nairobi, Kenya

S. NgigeDepartment of Medical Microbiology, College of Health Sciences, P. O. Box 19676, Nairobi, Kenya

C. R. A. BwiboDepartment of Medical Microbiology, College of Health Sciences, P. O. Box 19676, Nairobi, Kenya

P. C. MulegaDepartment of Medical Microbiology, College of Health Sciences, P. O. Box 19676, Nairobi, Kenya

J. F. KilonzoDepartment of Medical Microbiology, College of Health Sciences, P. O. Box 19676, Nairobi, Kenya

F. KibatiClinical Research Centre, Kenya Medical Research Institute, P. O. Box 54840, Nairobi, Kenya

J. OwateBaringo District Hospital, P. O. Box 21, Kabarnet, Kenya

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Abstract

A quick and suitable method for staining Leishmania donovani and other blood protozoa is described. The method, which could have wide applications, is a modification of Field's staining technique as used for malaria parasites. It uses the same stains in a different order. The method has been tried on smears containing malaria and trypanosomes, and the results were good. It is very cheap and requires no special apparatus or training.

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A rapid staining technique for Leishmania parasites in splenic aspirate smears

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A rapid staining technique for Leishmania parasites in splenic aspirate smears

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