Influence of soluble proteins from the salivary glands of ixodid ticks on the in-vitro proliferative responses of lymphocytes from BALB/c and C3H/HeN mice

Abstract

In the U.S.A., Borrelia burgdorferi, the causative agent of Lyme borreliosis, is transmitted to humans by the ticks Ixodes scapularis and I. pacificus. Tick modulation of host immunity is an important factor in tick transmission of such pathogens. The proliferative responses of lymphocytes from BALB/c and C3H/HeN mice exposed to the salivary-gland soluble proteins (SGSP) of I. scapularis, I. pacificus or Dermacentor andersoni were therefore compared in vitro. This produced the present report, the first to describe the effects of I. pacificus SGSP on the proliferative responses of a host's lymphocytes in vitro. The effects of four concentrations of SGSP from each tick species were evaluated with unstimulated, and concanavalin-A-stimulated lymphocytes of each mouse strain. The responses of lymphocytes from both mouse strains were significantly effected when exposed to SGSP derived from each tick species. Responses of the unstimulated lymphocytes to SGSP indicated that the proteins from I. pacificus suppressed in-vitro lymphocyte proliferation to a greater degree than those from the other species investigated. For the concanavalin-A stimulated cells, however, suppression of the proliferative responses was greatest for cells exposed to I. scapularis SGSP.