6
Views
35
CrossRef citations to date
0
Altmetric
Original Articles

NO2 Gas and NO2- Effects on Alveolar Macrophage Phagocytosis and Metabolism

, , , &
Pages 270-271 | Received 24 Aug 1972, Accepted 10 Dec 1972, Published online: 02 May 2013
 

Abstract

The in vitro elects of nitrogen dioxide (NO2) gas and nitrite ion (NO2 -) on the rates of entry and killing of Staphylococcus epi- dermidis and Pseυdomonas aeruginosa were measured in rabbit alveolar macrophages (AM) adherent to a glass surface. The metabolism of AM was studied in shaking cell suspensions with heat-killed epi- dermidis as test particles. Both NO2 and NO2 - (10 to 15 millimols) diminish bacterial entry and intracellular killing and increase 14CO2 production from 14C-1-glucose, 14C-6-glucose and 14C-1-pyruvate. In view of the potential role of H2O2 as an intracellular bactericidal agent, the effects of NO2 and NO2 -in catalase-dependent H2O2 metabolism were studied. Both NO2 and NO2 -(>.5 mM) react with catalase: H2O2 compound 1, as judged by diminution of formate oxidation and the sensitivity of AM catalase to aminotriazole inhibition.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.