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Abstract
Pentane and ethane, which arise during lipid peroxidation in vivo, were measured by gas chromatography in breath samples of rats fed for 8 weeks a vitamin E-deficient diet to which had been added 0 ,1 1 , or 40 IU vitamin E acetate per kg. Further lipid peroxidation was induced by exposure of individual rats to 1 ppm ozone for 60 min. Nonparametric statistical analysis of the data for pentane expired before exposure of rats to ozone gave α values (α = 2P) of 0.0006 when the 0 vitamin E group was compared with either of the vitamin Esupplemented groups. For ethane, comparison of the 0 vitamin E group with the groups supplemented with 11 and 40 IU vitamin E/kg of diet were 0.0294 and 0.0080, respectively. Alpha values < .05 were considered significant. After a 60-min exposure of rats to 1 ppm ozone, the paired t-test showed pentane to be significantly (P < .005) increased in only the rats fed the vitamin E-deficient diet.