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A gas chromatographic-mass spectrometric assay was developed to identify and measure the N-acetyl cysteine conjugate of cis-1,3-dichloropropene. The assay was used to show that individuals exposed to 1,3-dichloropropene vapor during field applications excrete this conjugate in their urine. The recoveries of the conjugate were correlated with the product of airborne concentrations and the duration of exposure (r = 0.83).
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