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Five species of Eucalyptus (E. bancroftii, E. nicholii, E. laevopinea, E. melliodora and E. grandis) have been brought into tissue culture on media devoid of coconut milk. One of these species (E. bancroftii) grows vigorously on a completely defined medium; the other four require casein hydrolysate for rapid growth.
Roots have been induced to regenerate from undifferentiated callus on media containing either 2,4-D or various combinations of IAA and kinetin.
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