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Abstract
1. Four methods of semen collecting that involved interruption of mating in two breeding ostrich pairs were tested: an artificial vagina was tested without promising results; the funnel method, in which a funnel was placed under the phallus of the tested male immediately after mating allowing semen drips to be collected; the vacuum method, using a turkey semen collector, inserted into the seminal canal; and the tube method, conducted by placing a test tube inside the seminal canal, allowing semen to enter by gravity. 2. For the funnel, vacuum and tube methods, respectively, average semen volume was 0·1 ± 0·02, 1·12 ± 0·22, and 0·58 ± 0·13 ml, sperm concentration was 0·66 ± 0·14, 2·35 ± 0·26, and 2·13 ± 0·27 × 109 cells/ml, and percentage of abnormal cells was 5·82 ± 1·79%, 4·68 ± 1·19%, and 7·09 ± 1·72%. 3. Semen characteristics varied throughout the reproductive season reaching peak concentration in June-July. 4. The vacuum method proved to be the most efficient and was a low stress, restraint-free method for collecting ostrich semen.