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Abstract
1. A polymerase-chain-reaction (PCR)-based test was adapted to sex the lesser rhea. Feathers instead of blood were used as the source of DNA.
2. The primers used allowed the assignment of sex by amplification of Z and W chromosome linked sequences.
3. This method proved to be safe, accurate and non-invasive.
4. The W chromosome linked fragment was sequenced and compared to other ratite sequences.
Acknowledgements
We wish to thank Dr J.L. Navarro and Dr C.N. Gardenal for helpful suggestions and Dr J. Rondan Dueñas for technical assistance. Zoo Córdoba allowed us to take tissue samples. This work was supported by the Agencia Nacional de Promoción Científica y Tecnológica (PICT No. 01-09682) to M.B. Martella.