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Abstract
1. Treating chicks, from 1 d or 5 weeks of age with ACTH three times weekly for 3 weeks depresses growth and causes adrenal hypertrophy at dose rates of 30 IU/kg or more, and depletion of adrenal cholesterol (> 10 IU/kg).
2. Treating chicks five times weekly at a dose rate of 30 IU/kg was as effective as a dose of 120 IU/kg three times weekly.
3. Plasma glucose and FFA concentrations of chicks treated thrice weekly with 120 IU ACTH/kg for 3 weeks were within the normal range.
4. Rhode Island Reds were more sensitive to ACTH than Light Sussex.
5. Adrenal cholesterol stores in normal chicks show significant variations with season.
