Chicken PRDX3 is required for proliferation of chicken embryo fibroblast cells

ABSTRACT

1. This experiment investigated the influence of chicken PRDX3 on cell proliferation in chick embryo fibroblast cells using PRDX3 knockdown technology.

2. A methyl thiazolyl tetrazolium (MTT) assay was performed to assess the effect of chPRDX3 knockdown on fibroblast proliferation. The antioxidant effect was investigated to determine if it directly mediated fibroblast cell proliferation.

3. To determine the role of chPRDX3 on cell proliferation, an siRNA mediated knockdown was performed in chick fibroblast cells using an in vitro assay. The proliferation of fibroblast cells transfected with siPRDX3 #3 and siPRDX3 Mix was significantly decreased after 48 h (P < 0.01). In addition, the knockdown of chicken PRDX3 suppressed cell proliferation through an increase in oxidative stress.

4. The results demonstrated that chPRDX3 is required for cell proliferation in chicken fibroblast cells. Such findings have important implications for the maintenance of chicken fibroblast cells.

KEYWORDS:

• Chicken embryo fibroblast cells
• peroxiredoxin 3
• proliferation

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was supported by the 2015-2019 RDA Fellowship Program of the National Institute of Animal Science, Rural Development Administration, Republic of Korea. This work also received support from the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01094401) of the Rural Development Administration, Republic of Korea.