ABSTRACT
1. The aim of this study was to investigate the effects of a combined probiotic product (Lactobacillus paracasei, L. plantarum, and L. rhamnosus) on egg quality, sulphur compounds in manure and serum biochemistry of second cycle hens.
2. A total of 48 White Leghorn hens, at 52- to 54 weeks of age, were treated in a completely randomised design with water containing 0 or 1.8375 × 1010 cfu/l of probiotics for 8 weeks.
3. Probiotic supplementation did not affect egg quality. Albumen height and Haugh units were in general lowest in week 2 and highest in week 6.
4. For manure and serum parameters, water with or without probiotics produced statistically similar effects. An interacting trend for increasing concentrations of methyl mercaptan in manure was noted for probiotics x week, and should be further investigated. The week of supplementation significantly affected hydrogen sulphide, dimethyl sulphide, and triglycerides.
Acknowledgments
The authors would like to acknowledge UAS Laboratories for kind contributions of the probiotics. The assistance provided by Sadia Naseem, Jacqueline Pisenti, and staffs at Hopkins Avian Facility, as well as statistical guidance from Neil Willits in the Department of Statistics at UC Davis, is greatly appreciated.
Disclosure statement
No potential conflict of interest was reported by the authors.