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Abstract
1. Effects of ageing and gonadal steroid hormones on pituitary prolactin (PRL) concentration in the chicken were investigated.
2. No difference was found in the qualitative electrophoretic patterns of the anterior pituitary homogenates at different ages and between the sexes.
3. Pituitary PRL concentrations in males were generally higher than in females.
4. Pituitary PRL concentrations in castrated immature cockerels were higher than in intact birds, while PRL concentration was depressed by the injection of testosterone propionate or oestradiol benzoate.
5. Prolactin concentration in intact immature cockerels was decreased by daily injection of testosterone or oestradiol for 3 weeks but not by injection of the hormones for 1 week.