Abstract
1. The proportions of granulosa cells containing one, two, or more nucleoli were determined in ovarian follicles of the domestic fowl (Gallus domesticus). Follicles ranged in size from 5 to 37 mm diameter.
2. The greatest proportion of cells (65%) contained one nucleolus. Cells containing two nucleoli were fewer (29%) and those containing more than two were rare (6%).
3. There was a tendency for the proportion of multinucleolar cells to decrease with increasing size of the follicle. Conversely there was a similar tendency for the proportion of cells with single nucleoli to increase with size of the follicle. These relationships are curvilinear.
4. Suitable linear models confirmed these observations (P < 0.005).
5. These results may provide an explanation for the previously reported progressive decrease in cellular DNA as the follicle increases in size.