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Abstract
1. Two forms, I and II, of β‐galactosidase [EC 3.2.1.23] from fowl spermatozoa were separated by Blue‐Sepharose CL‐6B chromatography.
2. The two forms of the enzyme yielded different pI values (4.1 and 4.7 for form I and 5.3 for form II).
3. Their gel filtration patterns were also different: form I resolved into two peaks with Mr about 65,000 and 80,000, whereas form II resolved into a single peak with an Mr of about 65,000.
4. Both forms had similar optimum pH, pH stability, thermal stability and Km values.
5. Differences between the characteristics of β‐galactosidase forms from spermatozoa and from seminal plasma suggest that the β‐galactosidases investigated in this study are spermatozoa‐specific.