Abstract
1. Assay conditions for measuring hepatic 5'‐deiodinase (5'D) activity at initial velocity, using reverse T3 as substrate, have been validated for adult chicken liver.
2. The characteristics of hepatic 5'D activity in adult chickens from lines selected for high (HW) and low (LW) juvenile body weight are similar to those in mammals and in the other birds that have been investigated.
3. Chickens from the HW line have significantly higher specific activity of hepatic 5'D, and thus potentially higher T3 production, than those from either the LW line or the Fl cross (HL) between the HW and LW lines.