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Original Articles

A Headspace Gas Chromatographic Method for the Determination of Total Ketone Body Concentration in Blood: Analysis of Samples Collected from Victims of Sexual Assault

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Pages 107-117 | Published online: 22 Nov 2013
 

ABSTRACT

Modification of the headspace gas chromatography method used for ethanol measurement allowed accurate quantitation of acetone in blood. Enzymatic conversion of 3-hydroxybutyrate to acetoacetate and subsequent conversion of the acetoacetate to acetone provided a specific, reproducible method for total ketone body determination. Analysis of blood obtained from sexual assault victims showed that the mean total ketone body concentration (310.1 μmol/L) for all cases was higher than the mean total for blood from control individuals (213.3 μmol/L). There was no significant difference in the total ketone body concentration between the sexual assault victims with visible signs of injury and those with no visible signs of injury (p>0.5). Consumption of ethanol by both groups of sexual assault victims inhibited production of all constituents of the ketone body pool.

RÉSUMÉ

Une modification de la méthode de dosage de l'éthanol par Chromatographie gazeuse, nous permet l'analyse quantitative précise de l'acétone dans le sang. La conversion enzymatique du 3-hydrobutyrate en acétoacétate et la transformation subséquente de l'acétoacétate en acétone constituent une méthode spécifique et reproductible pour la détermination des corps cétoniques. L'analyse du sang de victimes d'agression sexuelle a démontré que la concentration moyenne des corps cétoniques (310.1 μmole/L) dans tous les cas, était supérieure à la concentration sanguine moyenne des individus contrôles (213.3 μmole/L). Aucune différence significative n'a pu être démontrée entre les concentrations des corps cétoniques chez les victimes d'agression sexuelle avec blessures apparentes et chez celles ne démontrant aucune blessure apparente (p > 0.5). L'absorption d'éthanol par les deux groupes de victimes d'agression sexuelle a inhibé la production de tous les constituants de l'ensemble des corps cétoniques.

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