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Original Articles

A Rapid Color Test for Detection of Ethchlorvynol (PlacidylR) in whole Hemolyzed Blood

Pages 111-114 | Published online: 22 Nov 2013
 

ABSTRACT

A simple, rapid, inexpensive, and reliable method to presumptively screen for the presence of ethchlorvynol is proposed. The color test is based on the reaction of ethchlorvynol and diphenylamine in the presence of acid. The method is sensitive to low therapeutic concentrations in blood. Only 200 μL of a methanolic extract of blood, serum or plasma is required. The test can be performed semi-quantitatively and completed within 15 minutes.

RÉSUMÉ

Une méthode simple, rapide, peu coûteuse et fiable pour le criblage initial de l'ethchlorvynol est présentée. L'essai colorimétrique est basée sur la réaction de l'ethclorvynol et de la diphenhydramine en présence d'acide. Cette méthode peut détecter des concentrations sanguines d'ethchlorvynol qui se situent dans la partie inférieure de l'écart thérapeutique. L'essai ne requiert qu'un volume de 200 μL d'extrait méthanolique de sang, sérum ou plasma. Une analyse semi-quantitative peut être complétée en deçà de 15 minutes.

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