A Method for Obtaining Synaptonemal Complexes of Human Pachytene Oocytes

Weng Kong SungDepartments of Human Genetics and Development, Obstetrics and Gynecology and the Center for Reproductive Sciences of the International Institute for the Study of Human Reproduction, College of Physicians and Surgeons of Columbia University, 630 West 168th Street, New York, N.Y.10032, U.S.A.

Mosanobu KomatsuDepartment of Obstetrics and Gynecology, Hiroshima University School of Medicine, Kasumi 1–2–3, Hiroshima, Japan734

Georgiana JagielloDepartments of Human Genetics and Development, Obstetrics and Gynecology and the Center for Reproductive Sciences of the International Institute for the Study of Human Reproduction, College of Physicians and Surgeons of Columbia University, 630 West 168th Street, New York, N.Y.10032, U.S.A.

SUMMARY

A method is described for obtaining complete, well-spread synaptonemal complexes of human oocytes for light and electron microscopic examination. Preliminary karyotypes from each technique are presented. Examination of a cell from an aborted XXX fetal ovary revealed trivalent pairing of the X chromatids.

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A Method for Obtaining Synaptonemal Complexes of Human Pachytene Oocytes

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