Short term culture from solid tissues of fishes

SUMMARY

In fish cytogenetics the methods of chromosome preparations still need to be improved in order to obtain good metaphase figures for banding technics and more accurate analysis. In this paper we describe a very fast and simples culture method from solid fish tissues. Results of its application to different species as well as about 5-BrdU incorporation, both for chromosome banding and sister chromatid differentiation are reported. The data emphasized the advantage of this short term culture method wich provide excellent chromosome preparations suitables for basic and applied cytogenetic studies.