Cytological association between a knob on the short arm of chromosome 2 of maize and the latent-1 supergene

SUMMARY

C-banding technique was applied in some maize materials with the purpose of verifing cytogenetical association between chromosome 2S knob and the presence and expression of latent-1 supergene and also mitotic chromosome characterization. Materials chosen were IP 48-5-3 Cateto Assis Brasil, its isoline IP 48-5-3-lte-1 carring latent-1 factor and Michoacan 21-1-104 latent-1 donnor. C-band number obtained was 3.26 ± 0.69 for IP 48-5-3 Assis Brasil, 7.86±1.57 for IP-48-5-3-lte-1 and 8.43 ±1.12 for Michoacan 21-1-104. The mean of total heterochromatin amount values obtained and expressed as percentage were 4.07±0.46; 9.68±1.59 and 13.99±2.05, respectively, C-band was used because of good correspondence between heterochromatic knobs and positive C-bands. IP 48-5-3 lte-1 isoline and Michoacan 21 showed positive C-band on short arm of chromosome 2 and IP 48-5-3 Assis Brasil did not show any band in the same chromosome.

Key words:

• maize
• latent-1 supergene
• C-band
• mitosis