Advanced search
Publication Cover
Caryologia
International Journal of Cytology, Cytosystematics and Cytogenetics
Volume 52, 1999 - Issue 1-2
Journal homepage
97
Views
0
CrossRef citations to date
0
Altmetric
Original Articles

Detection of repetitive telomeric DNA on chromosomes in the cat by primed in situ labelling (PRINS)

W. Szolgyenyi Clinic for Poultry and Pet-BirdsCorrespondence[email protected]
,
K. Loschenberger Institute of Animal Breeding and Genetics, Veterinary University Vienna, Vienna, Austria
&
B. Mayr Institute of Animal Breeding and Genetics, Veterinary University Vienna, Vienna, Austria
Pages 93-95 | Received 06 Jul 1999, Accepted 13 Aug 1999, Published online: 18 Dec 2012
 
Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days

Abstract

We describe a rapid method for detection of telomeric DNA on chromosomes in the cat. Oligonucleotides were annealed to complementary sequences in fixed metaphase chromosomes of the cat and extended using Taq-DNA polymerase. Digoxygenin-11-dUTP was incorporated as a label for detection of the newly synthesized product. This method represents a quick and reliable alternative to conventional fluorescence in situ hybridization.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Download PDF

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.