A genetic physical map in river buffalo (Bubalus bubalis, 2n=50)

SUMMARY

This study reports the first entire genetic physical map in river buffalo chromosomes by ISH-techniques. Thirty-six loci (mostly mapped by FISH), of which 19 were expressed genes and 17 were DNA-segments (cosmid derived-microsatellites), were localized in specific chromosomes. At least one molecular marker from all 31 bovine syntenic groups was assigned to each river buffalo chromosome. This allowed tentative assignment of all bovine syntetic groups to river buffalo chromosomes, also in consideration of high degree of chromosome banding homologies between the two species and that all mapped loci in river buffalo chromosomes were at the same cattle homoeologous chromosomes and chromosome bands.

By adding markers from bovine U1, U3, U7, U10, U13, U16, U17, U25, U28 and U29 assigned to the river buffalo genome by the somatic cell hybrid technique, a total of 54 markers is assigned to river buffalo genome.

Key words:

• river buffalo
• gene mapping
• chromosomes
• in situ hybridization