Chromatin organisation and computer aided karyotyping of Triticum durum Desf. cv. Timilia

Abstract

The tribe Triticeae includes three cereal genera Secale, Hordeum and Triticum and because of their economical and agronomical importance, the relationships among species on the tribe have been extensively investigated. The wild wheat relatives are an important source of genetic variation for cultivated species and wheat is an important crop of the mediterranean region. Banding pattern of metaphase chromosomes and nuclear DNA content in root meristematic cells of an old sicilian landrace “Timilia” were determined. Microdensitometric evaluation of nuclear absorption at different thresholds of optical density indicates the organization of chromatin in the interphase nuclei. Chromosome morphometric data, karyotype simmetry, the TF% values and Syi indices were determined. The results are compared with the data of other durum wheat varieties as Capeiti and Simeto.

Key words:

• chromosome banding
• chromatin organization
• image analysis
• plant chromosomes
• Triticum
• cv. Capeiti
• cv. Simeto
• cv. Timilia
• wheat