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Abstract
The study of coffee species with conventional cytological techniques have been facing problems caused by its similar karyomorphology. Moreover the genus Coffea L. presents a great genetic homogeneity. New possibilities were brought up by the employment of cytomolecular techniques, based on in situ hybridization. In the present work we analyzed four wild diploid species (C. humilis, C. kapakata, C. sp. Moloundou and C. stenophylla) of the Coffee Germplasm Bank of IAC attempting to cytogenetic characterization. Chromosome banding with fluorescent staining DAPI and CMA3 and in situ hybridization with specific probes for rDNA sites pTa71 and pScT7 were used. Only for C. stenophylla we observed positive DAPI band. For C. kapakata and C. stenophylla the CMA3 positive bands present resemblance with the in situ hybridized sites in number, size and position. Although we observed few variations in the number and size of the evidenced chromosomal regions, these data are sufficient for karyotype distinguishing in the four studied species. The present results reinforce the importance of chromosome mapping by banding techniques and in situ hybridization in order to characterize species with similar karyomorphology.
