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Abstract
Amplified fragment length polymorphism (AFLP) marker and cytological analyses were employed to evaluate the genetic relationships and diversity of Chinese snakegourd. According to the cytological analyses, accessions 1, 2 and 3 were diploid (2n = 22), and accessions 4 to 12 were octaploid (2n = 88). Fourteen AFLP primer combinations generated a total of 645 reproducible loci among 12 Chinese snakegourd accessions studied, of which 628 loci were polymorphic with an average of 44.9 polymorphic bands per primer combination. Genetic similarities were obtained using Nei and Li similarity coefficients, and a dendrogram reflecting the relationship of 12 accessions was made using the unweighted pair-group method with arithmetic averages (UPGMA) clustering method. Based on the cluster analyses, 12 accessions were classified into two primary groups, in which group one included accessions 1, 2 and 3 that were diploid, and the other nine octaploid accessions formed group two. Analysis of molecular variance (AMOVA) revealed much greater variation among groups than within them. Meanwhile, some genotypes that might have great medicinal potential were also proposed according to their genetic relationships. These results provide exact evaluation of the relationships among Trichosanthes kirilowii accessions and give useful information for further investigation and utilization of this species.
Acknowledgments
The authors are grateful to the Agricultural Bureau of Changxing Country for providing us with three T. kirilowii accessions. We are also grateful to master Fan Tai-Wei for valuable advice on data analysis.
