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Original Articles

Determination of Fatty Acid Composition of Turfgrass by High‐Performance Liquid Chromatography

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Pages 53-61 | Received 06 Aug 2004, Accepted 31 May 2005, Published online: 20 Aug 2006
 

Abstract

The fatty acid composition of warm‐season turfgrasses is physiologically regulated by environmental changes, particularly temperature. Species or cultivars, which have increased degree of unsaturation, usually have a greater freeze resistance. Quantifying fatty acid composition not only can help understand the freeze‐resistant mechanism of warm‐season turfgrasses but also in practice develop a management strategy for better turf. A high‐performance liquid chromatography (HPLC) method was developed for this purpose. The method is based on the bromophenacyl reaction of fatty acids and separation on a C8 column with gradient elution. Information on limit of identification, linearity of quantification, response factor, and derivatization conditions is presented and discussed. The method presented is simple to apply and provides an alternative to the traditional gas chromatography (GC) method. The results of fatty acid composition from a field freezing‐resistance experiment are discussed.

Acknowledgments

This research is part of USDA‐CSREES project VA‐135660. Additional support was provided by the Virginia Turfgrass Council.

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