Mineralization of Three Organic Manures Used as Nitrogen Source in a Soil Incubated under Laboratory Conditions

Abstract

The rate and timing of manure application when used as nitrogen (N) fertilizer depend on N‐releasing capacity (mineralization) of manures. A soil incubation study was undertaken to establish relative potential rates of mineralization of three organic manures to estimate the value of manure as N fertilizer. Surface soil samples of 0–15 cm were collected and amended with cattle manure (CM), sheep manure (SM), and poultry manure (PM) at a rate equivalent to 200 mg N kg⁻¹ soil. Soil without any amendment was used as a check (control). Nitrogen‐release potential of organic manures was determined by measuring changes in total mineral N [ammonium‐N+nitrate‐N (NH₄ ⁺–N+NO₃ ⁻–N)], NH₄ ⁺–N, and accumulation of NO₃ ⁻–N periodically over 120 days. Results indicated that the control soil (without any amendment) released a maximum of 33 mg N kg⁻¹soil at day 90, a fourfold increase (significant) over initial concentration, indicating that soil had substantial potential for mineralization. Soil with CM, SM, and PM released a maximum of 50, 40, and 52 mg N kg⁻¹ soil, respectively. Addition of organic manures (i.e., CM, SM, and PM) increased net N released by 42, 25, and 43% over the control (average). No significant differences were observed among manures. Net mineralization of organic N was observed for all manures, and the net rates varied between 0.01 and 0.74 mg N kg⁻¹ soil day⁻¹. Net N released, as percent of organic N added, was 9, 10, and 8% for CM, SM, and PM. Four phases of mineralization were observed; initial rapid release phase in 10–20 days followed by slow phase in 30–40 days, a maximum mineralization in 55–90 days, and finally a declined phase in 120 days. Accumulation of NO₃ ⁻–N was 13.2, 10.6, and 14.6 mg kg⁻¹ soil relative to 7.4 mg NO₃ ⁻–N kg⁻¹ in the control soil, indicating that manures accumulated NO₃ ⁻–N almost double than the control. The proportion of total mineral N to NO₃ ⁻–N revealed that a total of 44–61% of mineral N is converted into NO₃ ⁻–N, indicating that nitrifiers were unable to completely oxidize the available NH₄ ⁺. The net rates of mineralization were highest during the initial 10–20 days, showing that application of manures 1–2 months before sowing generally practiced in the field may cause a substantial loss of mineralized N. The rates of mineralization and nitrification in the present study indicated that release of inorganic N from the organic pool of manures was very low; therefore, manures have a low N fertilizer effect in our conditions.

Keywords:

• Aerobic incubation
• immobilization
• manure nitrogen
• nitrification
• N transformation