Abstract
A laboratory incubation was conducted to evaluate nitrous oxide (N2O) production during nitrification and the effect of a nitrification inhibitor on N2O production from different profiles in a Japanese orchard Andosol. Soils were collected from five profiles: A1, A2, Bw1, Bw2, and BC. The soils were treated with ammonium sulfate at the rate of 200 mg N kg−1 with or without dicyandiamide (DCD) and incubated under aerobic conditions for 32 days. The net nitrification rate without DCD during the first 8 days was greater in the surface soils than in the subsurface soils. Accordingly, the surface soils showed a greater cumulative N2O production than the subsurface soils. Application of DCD significantly reduced the nitrification rate and thus N2O production from any depths of soils by 33.8 to 62.9%. Our study showed that substantial N2O was produced from the subsurface soil, although the amount was less than from the surface soils.