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Abstract
A greenhouse pot experiment was performed to determine the interactive effects of zinc (Zn) and nitrogen (N) application on growth, yield, and nutrient status in spinach. Two rates of Zn (150 and 500 mg Zn kg−1 dry soil) and five rates of N (0, 0.10, 0.20, 0.30, and 0.40 g N kg−1 dry soil) were arranged in a randomized complete block design with four replications. Phosphorus and K were applied at the rate of 0.20 g kg−1 dry soil in all treatment combinations. Main soil properties, concentrations of total and available Zn and N in soil, plant growth, yield, and nutrient status in shoots and roots were determined. The results showed that the greatest yields were achieved with the treatment combination of 150 mg Zn kg−1 and 0.20 g N kg−1. Nitrogen application had significant and positive effects on height and fresh- and dry-matter accumulation in spinach, whereas Zn application did not have significant influence. Soil total N concentration was significantly increased by the greatest N rate (0.40 g N kg−1) compared to other treatments, whereas that of leaves was not affected. Both nitrate levels in soil and leaves were significantly and positively affected by N application; however, they did not reach high levels. Although diethylenetriaminepentaacetic acid (DTPA)–extracted Zn was not significantly affected by applied N, total Zn concentration was significantly enhanced by the lower rate of N (0.10 g kg−1) in comparison to the control.