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ABSTRACT
Covalent capillary coatings of diazoresin (DR) and rapamycin (RAPA) were prepared by a layer-by-layer self-assembly. DR worked as the coupling agent. Under ultraviolet light, the corsslinking was carried out by covalent bonds between DR and RAPA. The covalent capillary coatings could suppress protein adsorption. The baseline separation of lysozyme (Lys), cytochrome c (Cyt-c), bovine serum albumin (BSA) and myoglobin (Mb) could be achieved within 17 minutes by using capillary electrophoresis (CE). The covalent capillary coatings exhibited good chemical stability and repeatability. In the synthesis, highly toxic moisture-sensitive silane has been replaced by DR. The method provides an environmentally friendly way to prepare the covalent capillary coating.
Funding
This work is financially supported by the National Natural Science Foundation of China (21574072, 21675091), the Taishan Young Scholar Program of Shandong Province (tsqn20161027), the Key Research and Development Project of Shandong Province (2016GGX102028, 2016GGX102039, 2017GGX20111), the Project of Shandong Province Higher Educational Science and Technology Program (J15LC20), the People's Livelihood Science and Technology Project of Qingdao (166257nsh, 173378nsh), the Innovation Leader Project of Qingdao (168325zhc), the Postdoctoral Scientific Research Foundation of Qingdao, and the First Class Discipline Project of Shandong Province.