Poly(hydroxyethyl methacrylate) inverse colloidal crystal membrane for efficient adsorption of lysozyme

Bing Yua Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, China;b State Key Laboratory of Bio-Fibers and Eco-Textiles, Qingdao University, Qingdao, ChinaView further author information

Shuai-Bing Guoa Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, ChinaView further author information

Xue-Xue Hana Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, ChinaView further author information

You-Qing Shena Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, ChinaView further author information

Hai-Lin Conga Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, China;b State Key Laboratory of Bio-Fibers and Eco-Textiles, Qingdao University, Qingdao, ChinaCorrespondence[email protected]
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Abstract

In this paper, we provide the adsorption behavior of lysozyme on the inverse colloidal crystal (ICC) membrane under different parameters (such as temperature, pH value, adsorption time). Besides, this also studied lysozyme adsorption behavior by changing the Hydroxyethyl methacrylate (HEMA) ratio of the ICC membrane. Furthermore, we found that ICC membranes containing Hydroxyethyl methacrylate have significantly improved lysozyme adsorption than ICC membranes without Hydroxyethyl methacrylate. With the increasing lysozyme concentration, the ICC membrane containing 50% HEMA showed an excellent selective adsorption effect. At the same time, we describe the adsorption mechanism of lysozyme on the ICC membrane containing HEMA. Finally, after ten adsorption-desorption cycles on the ICC membrane, the membranes adsorption effect on lysozyme has not significantly decreased.

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Funding

This work is financially supported via the National Natural Science Foundation of China (21675091, 21874078, 22074072), the Taishan Young Scholar Program of Shandong Province (tsqn20161027), and the High Level Discipline Project of Shandong Province.

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Poly(hydroxyethyl methacrylate) inverse colloidal crystal membrane for efficient adsorption of lysozyme

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Poly(hydroxyethyl methacrylate) inverse colloidal crystal membrane for efficient adsorption of lysozyme

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