A membrane filter technique for the concentration and detection of Salmonella from drinking water

A methodology for the concentration, detection and enumeration of Salmonella from drinking water by membrane filter technique is described. A single step filtration methodology for enteroviruses and Salmonella was not possible, due to the inactivation of Salmonella at conditions necessary for concentration of enteroviruses, i.e. pH 3.5 and 9.5. Enrichment in selenite cystine broth and incubation at 37°C followed by plating on brilliant green agar provided maximum recovery of Salmonella. Out of 21 drinking water samples in Delhi, India examined during March to May 1991, three were positive for Salmonella.

Key words:

• Drinking water
• Salmonella
• concentration
• MF technique and detection