LPS-INDUCED INFLAMMATORY CYTOKINE PRODUCTION WAS INHIBITED BY HYUNGBANGJIHWANGTANG THROUGH BLOCKADE OF NF-κB IN PERIPHERAL BLOOD MONONUCLEAR CELLS

Abstract

Hyungbangjihwangtang (HJT), a prescription of Sasang Constitutional Medicine, has been commonly used to treat diarrhea and edema of Soyangin in Korea. This study investigated the effect of HJT on lipopolysaccharide (LPS)-induced inflammatory cytokine production using peripheral blood mononuclear cells from the Soyangin. The inhibitory effect of HJT on LPS-induced inflammatory cytokine production was investigated using the enzyme-linked immunosorbent assay. Reverse transcriptase-polymerase chain reaction was used to investigate the interleukin (IL)-1β mRNA expression. The expression level of nuclear factor (NF)-κB was examined by Western blot. HJT significantly inhibited the IL-1β, IL-4, and tumor necrosis factor (TNF)-α production. The maximal inhibition rate of IL-1β, IL-4, IL-6, IL-8, and TNF-α production by HJT was 240.0 ± 48.8%, 78.4 ± 24.7%, 27.6 ± 10.6%, 20.7 ± 59.8%, and 113.0 ± 5.2%, respectively. HJT decreased the IL-1β mRNA expression. HJT also inhibited the activation of NF-κB. These results suggest a potential role of HJT as a source of anti-inflammatory agent for inflammatory diseases.

Keywords:

• Hyungbangjihwangtang
• inflammatory cytokine
• IL-1β mRNA
• NF-κB

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