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Original

In Vitro Characterization of Embryionic ST14A-Cells

, , , , &
Pages 1489-1501 | Published online: 07 Jul 2009
 

Abstract

The embryonic striatal temperature sensitive immortalized ST14A-cell line was characterized in vitro by immunocytochemistry when cultured at 33°C and at nonpermissive temperature of 39°C for up to 14 days. At 33°C in DMEM/10% FCS, cells proliferated, were extensively expressing the neural progenitor cell markers nestin and vimentin contrary to neuronal markers. However, when cultured at 39°C the proliferation was delayed and cells began to increase the expression of neuronal markers, followed by a decrease of nestin and vimentin. In serum-free medium the process of neuronal differentiation became more obvious, indicating the potential to use these cells for experimental restorative therapies.

Abbreviations
BSA=

bovine serum albumin

CNS=

central nervous system

DMEM=

Dulbecco's modified Eagle's medium

FCS=

fetal calf serum

MAP2=

microtubule associated protein 2

MAP5=

microtubule associated protein 5

NF160=

neurofilament 160

NF200=

neurofilament 200

NeuN=

neuron-specific nuclear protein

Abbreviations
BSA=

bovine serum albumin

CNS=

central nervous system

DMEM=

Dulbecco's modified Eagle's medium

FCS=

fetal calf serum

MAP2=

microtubule associated protein 2

MAP5=

microtubule associated protein 5

NF160=

neurofilament 160

NF200=

neurofilament 200

NeuN=

neuron-specific nuclear protein

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