MiR-623 links lncRNA RP11-89 and cyclin D1 to regulate the proliferation of glioblastoma cells

Abstract

Purpose

The tumorigenesis of bladder cancer has been proven to be related to the increased expression of lncRNA RP11-89, the participation of which in glioblastoma (GBM) is unknown. We predicted that RP11-89 could be targeted by miR-623, which targets cyclin D1. We then analyzed the role of RP11-89 in GBM.

Materials and methods

Samples of both GBM and paired non-tumor tissue were obtained from 58 GBM patients to analyze the expression of RP11-89 and miR-623 through RT-qPCR. The direct binding of miR-623 to RP11-89 was analyzed with RNA-RNA pull down. The role of RP11-89 and miR-623 in regulating each other’s expression was analyzed with overexpression assay. The role of RP11-89 and miR-623 in regulating the expression of cyclin D1 and GBM cell proliferation was analyzed by Western blot and BrdU assay, respectively.

Results

RP11-89 was expressed in high amounts in GBM, while miR-623 was expressed in low amounts in GBM. RP11-89 and miR-623 were not closely correlated, while miR-623 directly bound to RP11-89. RP11-89 and miR-623 showed no direct role in each other’s expression. RP11-89 suppressed the role of miR-623 in downregulating cyclin D1 and GBM cell proliferation.

Conclusions

Therefore, miR-623 may link lncRNA RP11-89 and cyclin D1 to regulate the proliferation of GBM cells.

Keywords:

• miR-623
• RP11-89
• cyclin D1
• glioblastoma
• GBM

Acknowledgements

Not applicable

Disclosure statement

The authors declare that they have no conflict of interests.

Authors contribution

Jiaqi Liao, Jinxian Xu concept, manuscript writing, editing and review

Kaiming Feng, Wentao Lai, Xiaohua Wen data collection and analysis, manuscript preparation

All authors have read and approve the submission of the manuscript.

Availability of data and materials

The analyzed data sets generated during the study are available from the corresponding author on reasonable request.

Ethical approval and consent to participate

All patients signed the written informed consent. All procedures were approved by Ganzhou People’s Hospital Ethics Committee. Procedures operated in this research were completed in keeping with the standards set out in the Announcement of Helsinki and laboratory guidelines of research in China.

Consent for publication

Not applicable.

Funding

The author(s) reported there is no funding associated with the work featured in this article.