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ABSTRACT
Natural hybrids of Rana epeirotica and Rana balcanica were studied by means of vertical acrylamide electrophoresis to confirm the various types of hybrids distinguished by the bioacoustic method. On the basis of four diagnostic loci (Ldh-1, Mdh-1, a muscle protein, and plasma albumin) at least three types of hybrids are distinguished. The most common type is the typical intermediate hybrid, which at all four loci displayed the two alleles of the parent species in the form of typical heterozygosity. Other types of hybrids give evidence of triploidy with either two genomes of R. balcanica and one of R. epeirotica, or one genome of R. balcanica and two of R. epeirotica. The possible role of the hybrid types is discussed.