Modulation of Afterhyperpolarization Evoked by Doublets and Increasing Number of Stimuli in Rat Motoneurons

ABSTRACT

The authors studied the influence of variable stimulation patterns on parameters of afterhyperpolarization (AHP) in rat spinal motoneurons using intracellular recording of antidromic action potentials. The action potentials analyzed were evoked by either (a) a single stimulus or pair of stimuli with gradually increasing interpulse intervals (IPI) of 5–20 ms or (b) an increasing number of stimulus pulses, from 1 to 4, at a constant IPI. It was demonstrated that modulations of AHP parameters after 2 pulses depended on the IPI, whereas the most significant changes in these parameters were found after the application of 2 or 3 stimuli in a series. The authors propose that changes in the AHP parameters have functional significance for motoneuronal firing pattern and therefore for motor unit force development during tetanic contractions.

Keywords:

• afterhyperpolarization
• motoneuron
• rat

ACKNOWLEDGMENTS

This research was supported by the Polish Ministry of Science and Higher Education Grant No. N N404 027035.

Notes

*Difference significant at p < 0.05: 2, 3, or 4 stimuli vs. 1 stimulus (the Wilcoxon test).

^#Difference significant at p < 0.05: 3 or 4 stimuli vs. 2 stimuli (the Wilcoxon test).