Cultural Studies In the Thelephoraceae and Related Fungi

SUMMARY

1.	The results of a general cultural investigation with species of the Thelephoraceae and related fungi are presented in tables I-III.
2.	In Cytidia solicina, (Fries) Burt the heterothallism is of the tetrapolar type, both the barrage reaction, described by Vandendries and Brodie (1933), and the false clamp connection reaction, described by Quintanilha (1935), occur on the community of the “B” factor.
3.	In Peniophora ludoviciana Burt the heterothallism is of the bipolar type. The cells of the haploid hyphae are always multinucleate. The cells of the diploid hyphae are initially multinucleate but finally binucleate clamp bearing cells develop. In this species both the haploid and the diploid mycelia produce basidiospore fructifications in culture. The young haploid basidia are binucleate and a fusion of nuclei occurs. The occurrence of a fusion nucleus in the haploid phase provides additional evidence of the origin of homothallic species from heterothallic ancestors.
4.	In Peniophora gigantea (Fries) Massee, Coniophora cerebella Pers. and an unidentified species of Corticium, no. 526, the cells of the mycelium of polysporous cultures are multinucleate. Abnormal clamp connections are produced in connection with cell division in the multinucleate cells.