Nutrient Requirements for two Species of Aquatic Hyphomycetes

SUMMARY

1.	Attguillospora longissinta and A. gigantea were found to grow-best at 19° C and 28° C, respectively, in standing culture and both at 28° C in shake culture, between pH 6.5 and pH 6.7, and to attain maximum dry weight in between three and four weeks in a liquid medium composed of glucose, mineral salts and yeast extract.
2.	The two species were found to be heterotrophic for thiamin and a carbohydrate source only. They are able to utilize nitrate as a source of nitrogen and sulphate as a source of sulphur. The usual mineral salts are presumably required as well.
3.	The organisms can satisfactorily utilize a wide range of carbohydrates that includes starch, cellulose, cellobiose, sucrose, maltose, glucose, galactose, mannose, and xylose. Anguillospora gigantea was also found to grow well on arabinose and made only poor growth on pectin. A. longissinta, on the other hand, did not grow well either on arabinose or on pectin.