The Effect of Added B-Vitamins on the Growth and Ester Production of Hansenula Anomala (Hansen) Sydow

SUMMARY

Investigations were conducted to determine the relation of nine vitamins of the B-complex and six trace elements to growth and ethyl acetate production by Hansenula anomala (Hansen) Sydow. Results of these investigations were as follows:

1.	Twenty-four hours after inoculation into medium with added vitamins there was a decided increase in growth over the cultures containing no added vitamins. The greatest difference between cultures containing no vitamins and cultures containing all vitamins was apparent at about 48 hours. After 96 hours the amount of growth in these cultures was essentially equal. Addition of single vitamins resulted in similar growth differences. On the basis of these results it was concluded that this yeast has no vitamin deficiency for the nine vitamins of the B-complex that were tested.
2.	The yields of ethyl acetate were highest when both vitamins and trace elements were added to the medium. An increase in the concentration of trace elements gave an increase in the amount of ester synthesized.
3.	There was a greater increase in ethyl acetate production when trace elements alone were added to the medium than when vitamins were added alone.
4.	An interaction between vitamins, trace elements, growth, and ester production by this organism is indicated by these studies.