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SUMMARY
The formation and maturation of the sexual stage of Cochliobolus sativus in laboratory cultures were greatly affected by environmental factors. Perithecia failed to develop on media from which plant parts were excluded. The agar media served only as moisture sources; the nutritive elements in the media were non-specific in function except as buffers to maintain a pH conducive to perithecial formation. The incubation of cultures at 24° for seven days followed by 20° for 14 days, and on media of pH 4 to 6, favored the development of mature perithecia. Although perithecia formed under all light conditions, sunlight apparently inhibited to some extent the delimitation of ascospores.
Random-spore analysis for conidial color (white and olivaceous) and mating type (A and a) indicated that the characters were not linked. Probably each was conditioned by an allelic gene pair.
The authors are indebted to Dr. B. J. Sallans for his helpful advice and the critical review of the manuscript.