SUMMARY
Two species of Gasteromycetes, Phallus ravenelii and Crucibulum levis were studied in liquid culture. The utilization of nutrients was determined by average mycelial dry weight of 2–6 replicates. Optimum growth for both species occurred at pH 5.5.
Phallus ravenelii and Crucibulum levis were able to utilize various carbon sources when grown on modified Lindeberg's medium. Phallus ravenelii gave excellent yields on xylose, mannitol, dextrin, fructose, cellobiose, mannose, glycogen, glucose, starch, and maltose, but growth was poor in media containing trehalose, sorbose, ribose, arabinose, sucrose, lactose, and raffinose. Crucibulum levis grew most rapidly on glucose, cellobiose, dextrin, starch, maltose, glycogen, mannose, and fructose. Xylose, trehalose, galactose, arabinose, sucrose, ribose, lactose, mannitol, raffinose and sorbose supported very little growth.
Yields of mycelium were high on inorganic ammonium-nitrogen and ammonium-nitrate. Crucibulum levis grew favorably on but one amino acid, isoleucine. Six amino acids gave excellent yields of Phallus ravenelii: alanine, glutamic acid, serine, arginine, asparagine, and aspartic acid. Phenylalanine, leucine, glycine, and isoleucine produced good growth responses in the same species.
Both Phallus ravenelii and Crucibulum levis were heterotrophic for thiamine.