SUMMARY
Arginyl-transfer ribonucleic acid (tRNA) species from Neurospora crassa wild-type strain 74-OR23-1A were studied by reversed-phase chromatographic columns. Arginyl-tRNA species from the nucleus, mitochondrion, and cytoplasm were compared as eluted from the column, and exhibited different chromatographic profiles. No unique species of arginyl-tRNA appeared in the various cell fractions, but rather different amounts of the minor species existed in the various cell components in contrast to that of whole-cell extracts. A characterization of nuclear and mitochondrial arginyl-tRNA synthetases showed that both differ in elution profiles and charging properties, which suggested that Neurospora crassa possessed both a nuclear and mitochondrial arginyl-tRNA synthetase (s).