Alpha-Galactosidase from Pichia Guilliermondii

SUMMARY

Examination of a variety of species of molds and yeasts, including those of the genus Pichia, has revealed significant production of the hydrolytic enzyme α-galactosidase in Pichia guilliermondii. Release of the enzyme in this ascosporogenous yeast was strongly affected by certain substrates such as melibiose and lactose, and soybean products, including casitone, soytone, and soybean-oil meal. Maximal α-galactosidase was detected in media containing lactose and the soytone product at pH 7. Enzyme production was stimulated by addition of metal salts to the culture broth.