Phenoloxidase and Peroxidase Activities in Zone Lines of Phellinus Weirii

SUMMARY

Phenoloxidase and peroxidase were extracted from pigmented zones (zone lines) which were formed on agar media between two Phellinus weirii isolates or by one P. weirii isolate in response to the presence of an antagonist. Activity of enzymes from zone lines was compared with that of enzymes from mycelial tissues adjacent to the zone lines by isoelectric focusing on Polyacrylamide gels. Zone lines produced stronger phenoloxidase and peroxidase reactions than did adjacent tissues. At least three phenoloxidases and as many as six peroxidase isozymes were detected in zone lines, some of which may have been induced or caused by the antagonists. Phenoloxidase and peroxidase were observed also in the zone lines formed by P. weirii in colonized Douglas-fir wood, but not in adjacent tissues.