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ABSTRACT
In an attempt to find new mutations in the aflatoxin pathway, mutant strains that accumulate known aflatoxin precursors (norsolorinic acid, averufin, and versicolorin A) were subjected to a second mutagenic treatment. Eighty presumptive mutants were isolated based on mycelial color differences. The presumptive mutants were grown in two liquid defined media and the chromatographic profiles of mycelial extracts were compared after thin layer chromatography (tlc) in two solvent systems. Six presumptive mutants produced spots not observed in controls on tic plates viewed under long-wave ultraviolet light. One of these newly isolated mutants produced at least six compounds not identified in the parental strain. Mycelial extracts of this new mutant, four previously characterized anthraquinone-accu- mulating mutants, and wild type Aspergillus parasiticus were compared by dry column chromatography. Each of these strains gave a unique dry column chromatographic pattern.