Selective Media for Recovery of Cylindrocladium and Fusarium Species from Roots and Stems of Tree Seedlings

ABSTRACT

Root and soil-level stem sections of diseased forest tree seedlings were placed on glucoselima bean rose bengal agar (GLBRA) to determine the efficacy of the medium for recovery of Cylindrocladium species. Yeast extract was substituted for lima bean agar in the medium to determine its effect on Cylindrocladium isolation. The GLBRA medium allowed for excellent recovery, growth, and microsclefotial production of Cylindrocladium species from all seedlings tested, and effectively suppressed contaminating fungi and bacteria. The use of yeast extract reduced recovery of Cylindrocladium species, but greatly enhanced isolation of Fusarium species.

Key Words:

• fungal isolation media
• Cylindrocladium
• Fusarium