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ABSTRACT
To initiate studies on the developmental biochemistry of oogenesis, we have developed a mating system which produces highly uniformly differentiating cultures of female Achlya ambisexualis. This method involves natural matings, in shake cultures, of male and female strains possessing different colony morphologies. We have identified three specific developmental stages to act as markers for future biochemical analyses. Nuclear positioning during oogenesis was examined using epi-fluorescent microscopy.