Selection of Hypercellulolytic Mutants of Trichoderma Reesei Based on Resistance to Nystatin

John SchimentiDepartment of Biochemistry and Microbiology, Cook College, New Jersey Agricultural Experiment Station, Rutgers University, New Brunswick, New Jersey08903

T. GarrettDepartment of Biochemistry and Microbiology, Cook College, New Jersey Agricultural Experiment Station, Rutgers University, New Brunswick, New Jersey08903

Bland S. MontenecourtDepartment of Biochemistry and Microbiology, Cook College, New Jersey Agricultural Experiment Station, Rutgers University, New Brunswick, New Jersey08903

Douglas E. EveleighDepartment of Biochemistry and Microbiology, Cook College, New Jersey Agricultural Experiment Station, Rutgers University, New Brunswick, New Jersey08903

ABSTRACT

Nystatin-resistant mutants of Trichoderma reesei were isolated, and several derived from the wild strain showed increased production of cellulase. Thus, the concept of gaining greater enzyme release through interference with membrane function was confirmed. Certain nystatin-resistant mutants derived from the hypercellulolytic strain RUT-C30 also showed increased cellulase yields, but these strains proved unstable and reverted to giving basal enzyme yields.

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Selection of Hypercellulolytic Mutants of Trichoderma Reesei Based on Resistance to Nystatin

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Selection of Hypercellulolytic Mutants of Trichoderma Reesei Based on Resistance to Nystatin

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